PAOL couples an exclusion chromatography and different detectors, static and dynamic light scattering, refractive index and absorbance. PAOL combines the separation of macromolecules and their characterization. See thetechnical and scientist description. It allows:
- Quantifying the homogeneity of the preparations.
- Determining the stoichiometry of complexes, including for complex systems associating protein-detergent, protein-RNA, protein-polymer. It is a method often appropriated for studying interactions of membrane protein and/or glycosylated proteins.
See more at:
http://www.isbg.fr/biophysics-characterisation/protein-analysis-on-line-paol/