FRISBI’s centres offer a complete set of platforms (techniques) for the integrated structural biology research process. Explore the platforms by stage of the process.
Sample Preparation
Sample preparation is not only a key issue for structural and molecular biology studies, but concerns Life Sciences development as a whole. FRISBI offer state-of-the-art infrastructures for the expression and production of biomolecules and their complexes in prokaryotic and eukaryotic system.
Amino acid analysis/ Nter sequencing
FRISBI centers offering this: IBS – ISBG
Bacterial expression
FRISBI centers offering this: AFMB, IGBMC/CBI-Biostructure, IBS – ISBG
Camelid nanobody production
FRISBI centers offering this: AFMB
Cell free expression
FRISBI centers offering this: IBS – ISBG
Gene Tagging
FRISBI centers offering this: IGBMC/CBI-Biostructure
Insect cells expression
FRISBI centers offering this: AFMB, IGBMC/CBI-Biostructure, PARIS-SACLAY
Isotopic labelling
FRISBI centers offering this: CBS, IBS – ISBG
Library based construct screening
FRISBI centers offering this: IBS – ISBG
Mammalian expression
FRISBI centers offering this: AFMB, PARIS-SACLAY, IGBMC/CBI-Biostructure
Mass spectrometry
FRISBI centers offering this: IBS – ISBG
Membrane Protein Purification Platform
FRISBI centers offering this: IBS – ISBG
Purification
FRISBI centers offering this: CBS, IGBMC/CBI-Biostructure
Yeast expression
FRISBI centers offering this: PARIS-SACLAY, IGBMC/CBI-Biostructure
Biophysical characterisation
FRISBI offers a large panel of state-of-the-art equipment and expertise in biochemistry and biophysics to perform quality control and functional analysis on protein samples.
Analytical Ultra Centrifugation
FRISBI centers offering this: IGBMC/CBI-Biostructure, IBS – ISBG
Bio-Layer Interferometry (BLI)
FRISBI centers offering this: AFMB, IGBMC/CBI-Biostructure, PARIS-SACLAY
Calorimetry
FRISBI centers offering this: AFMB, CBS, IGBMC/CBI-Biostructure, PARIS-SACLAY
Circular Dichroism
FRISBI centers offering this: CBS, IGBMC/CBI-Biostructure
Flow-Induced Dispersion Analysis (FIDA)
FRISBI centers offering this: IGBMC/CBI-Biostructure
High pressure
FRISBI centers offering this: IBS – ISBG
MALS
FRISBI centers offering this: AFMB, IGBMC/CBI-Biostructure, IBS – ISBG
Microscale thermophoresis
FRISBI centers offering this: AFMB, IGBMC/CBI-Biostructure, PARIS-SACLAY
Selection of specific alphaRep binders
FRISBI centers offering this: PARIS-SACLAY
SPR
FRISBI centers offering this: IBS – ISBG
switchSense
FRISBI centers offering this: PARIS-SACLAY
Thermal Shift Assay
FRISBI centers offering this: IBS – ISBG, AFMB, CBS, IGBMC/CBI-Biostructure
Crystallisation
Crystallisation of protein molecule is a crucial step during the course of X-ray structure determination.
Crystallisation
FRISBI centers offering this: CBS, PARIS-SACLAY, AFMB
Crystallisation under anaerobic conditions
FRISBI centers offering this: IBS – ISBG
High Throughput Crystallisation
FRISBI centers offering this: IGBMC/CBI-Biostructure, IBS – ISBG
High throughput membrane protein crystallisation
FRISBI centers offering this: IBS – ISBG
Structural analysis
FRISBI offers acces to a range of state of the art structural biology technologies and integrative biological approaches and expertise.
Cryobench
FRISBI centers offering this: IBS – ISBG
Cryo Electron Microscopy
FRISBI centers offering this: AFMB, IGBMC/CBI-Biostructure, PARIS-SACLAY
Crystallography
FRISBI centers offering this: PARIS-SACLAY
Electronic spectroscopy
FRISBI centers offering this: PARIS-SACLAY
Electron microscopy
FRISBI centers offering this: CBS, IBS – ISBG
EPR
FRISBI centers offering this: PARIS-SACLAY
FIP beamline
FRISBI centers offering this: IBS – ISBG
FTIR Fourier Transform InfraRed spectroscopy
FRISBI centers offering this: PARIS-SACLAY
In-house X-ray cristallography
FRISBI centers offering this: CBS
Light microscopy
FRISBI centers offering this: IBS – ISBG, IGBMC/CBI-Biostructure
Liquid State NMR
FRISBI centers offering this: CBS
NMR
FRISBI centers offering this: IGBMC/CBI-Biostructure, IBS – ISBG, PARIS-SACLAY
Optical Super resolution Spectroscopy
FRISBI centers offering this: PARIS-SACLAY
Resonance Raman spectroscopy
FRISBI centers offering this: PARIS-SACLAY
Small Angle X-ray Scattering
FRISBI centers offering this: CBS
Super resolution microscopy
FRISBI centers offering this: IGBMC/CBI-Biostructure
X-ray crystallography
FRISBI centers offering this: AFMB
Bioinformatics
In the framework of integrated structural biology, the goal of bioinformatics is not only to facilitate the determination of a protein’s 3D structure, but also to understand its role(s) in the complex, dynamic networks that are responsible for cellular or organismal functions. This requires dedicated computational systems that can handle and store large volumes of heterogeneous data and that can quickly process queries between the different data resources and efficiently extract relevant knowledge.
Bioinformatics
FRISBI centers offering this: AFMB
Structural Bioinformatics
FRISBI centers offering this: CBS
CENTERS
FRISBI is a distributed infrastructure comprising 5 centers
Illkirch-Graffenstaden
Grenoble
Montpellier
Marseille